Overview

  • Product nameAnti-BAT3 antibody
    See all BAT3 primary antibodies
  • Description
    Rabbit polyclonal to BAT3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1076-1125 (ARPLTSPESL SRDLEAPEVQ ESYRQQLRSD IQKRLQEDPN YSPQRFPNAQ) of Human BAT3 (NP_004630).

  • Positive control
    • MCF7 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab116325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Predicted molecular weight: 119 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionChaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Also involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
  • Sequence similaritiesContains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
    In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex.
  • Cellular localizationCytoplasm > cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410045D21Rik antibody
    • AA408914 antibody
    • BAG 6 antibody
    • BAG family molecular chaperone regulator 6 antibody
    • BAG-6 antibody
    • BAG6 antibody
    • BAG6_HUMAN antibody
    • BAT 3 antibody
    • BCL2-associated athanogene 6 antibody
    • D17H6S52E antibody
    • D6S52E antibody
    • G3 antibody
    • HLA B associated transcript 3 antibody
    • HLA-B associated transcript 3 antibody
    • HLA-B associated transcript-3 antibody
    • HLA-B-associated transcript 3 antibody
    • large proline rich protein BAG6 antibody
    • Large proline rich protein BAT3 antibody
    • Large proline-rich protein BAG6 antibody
    • large proline-rich protein BAT3 antibody
    • Protein G3 antibody
    • Protein Scythe antibody
    • Scythe antibody
    • Scythe, homolog of Xenopus antibody
    see all

Anti-BAT3 antibody images

  • Anti-BAT3 antibody (ab116325) at 0.125 µg/ml + MCF7 cell lysate at 10 µg

    Predicted band size : 119 kDa

References for Anti-BAT3 antibody (ab116325)

ab116325 has not yet been referenced specifically in any publications.

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