Recombinant
RabMAb

Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549)

Overview

  • Product name
    Anti-BAT5 antibody [EPR15463] - C-terminal
  • Description
    Rabbit monoclonal [EPR15463] to BAT5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BAT5 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: O95870

  • Positive control
    • Caco-2, 293, HT29 and BxPC-3 cell lysate. BxPC-3 cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15463
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 63 kDa).

Target

Images

  • All lanes : Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549) at 1/1000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : BxPC-3 cell lysate
    Lane 4 : HT-29 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-BAT5 antibody [EPR15463] - C-terminal (ab185549) at 1/1000 dilution

    Lane 1 : BxPC-3 cell extract
    Lane 2 : BxPC-3 cell extract with immunizing peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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