• Product nameAnti-BATF antibody
    See all BATF primary antibodies
  • Description
    Rabbit polyclonal to BATF
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 36-85 (NRIAAQKSRQ RQTQKADTLH LESEDLEKQN AALRKEIKQL TEELKYFTSV) of human BATF (NP_006390).

  • Positive control
    • Transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab86965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionAP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.
  • Tissue specificityExpressed at highest levels in lung, and at lower levels in placenta, liver, kidney, spleen, and peripheral blood. Detected in SW480 colorectal cancer cell line and several hematopoietic tumor cell lines, including Raji Burkitt's lymphoma. Strongly expressed in mature B- and T-lymphocytes. Also expressed in moderate levels in lymph node and appendix and at low levels in thymus and bone marrow (PubMed:10777209).
  • Sequence similaritiesBelongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.
  • Cellular localizationNucleus. Cytoplasm. Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor B antibody
    • B ATF antibody
    • B-ATF antibody
    • B-cell-activating transcription factor antibody
    • Basic leucine zipper transcription factor like antibody
    • Basic leucine zipper transcriptional factor ATF like antibody
    • Basic leucine zipper transcriptional factor ATF-like antibody
    • Batf antibody
    • BATF_HUMAN antibody
    • BATF1 antibody
    • SF HT activated gene 2 protein antibody
    • SF-HT-activated gene 2 protein antibody
    • SFA 2 antibody
    • SFA-2 antibody
    • SFA2 antibody
    see all

Anti-BATF antibody images

  • Anti-BATF antibody (ab86965) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BATF antibody (ab86965)

ab86965 has not yet been referenced specifically in any publications.

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