Overview

  • Product nameAnti-BATF3 antibody
    See all BATF3 primary antibodies
  • Description
    Rabbit polyclonal to BATF3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 1-50 (MSQGLPAAGS VLQRSVAAPG NQPQPQPQQQ SPEDDDRKVR RREKNRVAAQ) of Human BATF3 (NP_061134).

  • Positive control
    • HT1080 cell lysate. IHC-P: FFPE human lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab98141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceBATF3 belongs to the bZIP family and contains 1 bZIP domain. Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12-O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 21 kD small nuclear factor isolated from T cells antibody
    • 21 kDa small nuclear factor isolated from T-cells antibody
    • ATF like 3 antibody
    • B-ATF-3 antibody
    • basic leucine zipper transcription factor, ATF-like 3 antibody
    • BATF3 antibody
    • FLJ36352 antibody
    • FLJ37535 antibody
    • hCG 39202 antibody
    • JDP1 antibody
    • Jun dimerization protein 1 antibody
    • Jun dimerization protein p21SNFT antibody
    • JUNDM1 antibody
    • SNFT antibody
    see all

Anti-BATF3 antibody images

  • IHC image of BATF3 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab98141, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-BATF3 antibody (ab98141) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 14 kDa
    Gel concentration: 10-20%

References for Anti-BATF3 antibody (ab98141)

ab98141 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Sample Human Tissue sections (Lymph node)
Specification Lymph node
Permeabilization No
Fixative Acetone
Username

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Verified customer

Submitted Jul 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"