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Synthetic peptide corresponding to Human Bax aa 43-61 (internal sequence).
This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Our Abpromise guarantee covers the use of ab10813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 21 kDa.|
ab10813 immunoprecipitating Bax. 2mg of human neuroblastoma whole cell lysate was incubated with ab10813 at a 1/1000 dilution for 16 hours and then precipitated with protein A. An HRP polyclonal antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by David MacDonald on 6 February 2006.