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Immunoprecipitation - Bax antibody (ab10813)
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Immunocytochemistry/ Immunofluorescence - Bax antibody (ab10813)
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Product Name
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Bax antibody
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See all Bax antibodies (21)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bax
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Immunogen
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Synthetic peptide, corresponding to amino acids 43-61 of Human Bax.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Tested applications
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200.
IHC-P: 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/1000.
WB: Use at an assay dependent dilution. Detects a band of approximately 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Normal Breast Tissue.
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Cellular localization
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Cell Membrane (alpha isoform) and Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Relevance
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Bcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.09% Sodium Azide
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bax antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bax antibody images, references, products related to ab10813 and other tools.
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Bax antibody images:
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 Immunoprecipitation - Bax antibody (ab10813)
ab10813 immunoprecipitating Bax. 2mg of human neuroblastoma whole cell lysate was incubated with ab10813 at a 1/1000 dilution for 16 hours and then precipitated with protein A. An HRP polyclonal antibody was used as the secondary antibody. This image is courtesy of an Abreview submitted by David MacDonald on 6 February 2006.
See Abreview
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Immunocytochemistry/ Immunofluorescence - Bax antibody (ab10813)
ab10813 (1/200) staining Bax in assynchronous Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5%Triton X-100/PBS and counterstained with DAPi in order to highlight the nucleus. Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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Search PubMed (MEDLINE) for references to Bax
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Bax antibody - more information
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Customer reviews (feedback) regarding Bax antibody |
Customer FAQs regarding Bax antibody |
Protocols for Bax antibody |
Price and availability of products related to Bax antibody |
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Bax antibody - related products:
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Bax antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bax antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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