Overview

  • Product nameAnti-Bax antibody
    See all Bax primary antibodies
  • Description
    Rabbit polyclonal to Bax
  • Specificityab62641 detects endogenous levels of total Bax protein.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Bax (C terminal). The exact sequence is proprietary. Derived from around the phosphorylation site of threonine 167
    Sequence:

    F-G-Tp-P-T


    Database link: Q07812

  • Positive control
    • HuvEc cells, LOVO cells, HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab62641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
ELISA 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • FunctionAccelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificityExpressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationCytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Anti-Bax antibody images

  • Immunofluorescence analysis of LOVO cells, staining Bax with ab62641 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • Immunofluorescence analysis of HuvEc cells, staining Bax with ab62641 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • All lanes : Anti-Bax antibody (ab62641) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunizing peptide


    Predicted band size : 21 kDa

References for Anti-Bax antibody (ab62641)

ab62641 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.


We have two antibodies tested in Bax in ICC/IF as ab10813 and ab62641.


http://www.abcam.com/index.html?datasheet=10813 (or use the following: http://www.abcam.com/index.html?datasheet=10813). ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"