Anti-Bax antibody [SP47] (ab81083)

Overview

• Product name
  Anti-Bax antibody [SP47]
  See all Bax primary antibodies
• Description
  Rabbit monoclonal [SP47] to Bax
• Tested applications
  Suitable for: Flow Cyt, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow
• Immunogen

  Synthetic peptide corresponding to Human Bax (internal sequence).
  Database link: Q07812

• Positive control
  • Human breast, cervix, esophagus, prostate, skin, tonsil normal tissue. Skin and lung squamous cell carcinoma, Hodgkin's lymphoma.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.60
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, PBS
• Concentration information loading...
• Purity
  Affinity purified
• Purification notes
  Purified by protein A/G chromatography
• Clonality
  Monoclonal
• Clone number
  SP47
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Apoptosis marker and proteins ELISA kits

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Apoptotic Markers
  • Bcl 2 family

  • Cancer
  • Cell Death
  • Apoptosis
  • Metabolism

Associated products

• Related Products
  • ABT-737 (ab141336)
  • 2-Methoxyantimycin A3 (ab141523)
  • Z36 (ab141757)
  • Recombinant Human Bax protein (Tagged) (ab85157)

Applications

Target

• Function
  Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
• Tissue specificity
  Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
• Sequence similarities
  Belongs to the Bcl-2 family.
• Domain
  Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
• Cellular localization
  Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.

• Target information above from: UniProt accession Q07812 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 280730 Cow
  • Entrez Gene: 581 Human
  • Entrez Gene: 12028 Mouse
  • Entrez Gene: 24887 Rat
  • Omim: 600040 Human
  • SwissProt: O02703 Cow
  • SwissProt: Q07812 Human
  • SwissProt: Q07813 Mouse

  • SwissProt: Q63690 Rat
  • Unigene: 624291 Human
  • Unigene: 19904 Mouse
  • Unigene: 10668 Rat

  see all
• Alternative names
  • Apoptosis regulator BAX antibody
  • BAX antibody
  • Bax-protein antibody

  • BAX_HUMAN antibody
  • BAXA antibody
  • Baxdelta2G9 antibody
  • Baxdelta2G9omega antibody
  • Baxdelta2omega antibody
  • Bcl-2-like protein 4 antibody
  • BCL2 associated X protein antibody
  • BCL2 associated X protein omega antibody
  • BCL2 associated X protein transcript variant delta2 antibody
  • Bcl2-L-4 antibody
  • BCL2L4 antibody
  • membrane isoform alpha antibody

  see all

Protocols

• Flow cytometry protocols
• Immunohistochemistry protocols