Anti-Bax antibody [SP47], prediluted (ab81084)

Overview

  • Product nameAnti-Bax antibody [SP47], prediluted
    See all Bax primary antibodies
  • Description
    Rabbit monoclonal [SP47] to Bax, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Bax (internal sequence).
    Database link: Q07812

  • Positive control
    • Human breast, cervix, esophagus, prostate, skin, tonsil normal tissue. Skin and lung squamous cell carcinoma, Hodgkin's lymphoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab81084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAccelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificityExpressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationCytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Anti-Bax antibody [SP47], prediluted images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human esophagus tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervix tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin squamous cell carcinoma tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung squamous cell carcinoma tissue staining Bax with ab81084, pre-diluted.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue staining Bax with ab81084, pre-diluted.

References for Anti-Bax antibody [SP47], prediluted (ab81084)

ab81084 has not yet been referenced specifically in any publications.

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