Anti-Bax inhibitor 1 antibody (ab18852)

Overview

  • Product nameAnti-Bax inhibitor 1 antibody
    See all Bax inhibitor 1 primary antibodies
  • Description
    Rabbit polyclonal to Bax inhibitor 1
  • Specificityab18852 recognises Bax inhibitor.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of Bax inhibitor 1.

  • Positive control
    • rat testis tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 26 kDa.

Target

Anti-Bax inhibitor 1 antibody images

  • Anti-Bax inhibitor 1 antibody (ab18852) at 1 µg/ml + rat testis tissue lysate

    Predicted band size : 26 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab18852 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18852, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Bax inhibitor 1 antibody (ab18852)

ab18852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"