The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 79 kDa).
FunctionProbable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.
Involvement in diseaseBardet-Biedl syndrome 12
Sequence similaritiesBelongs to the TCP-1 chaperonin family. BBS12 subfamily.
Cellular localizationCell projection, cilium. Located within the basal body of the primary cilium of differentiating preadipocytes.
Marion V et al. Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation. Proc Natl Acad Sci U S A106:1820-1825 (2009).
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