Customer reviews and FAQs

Overview

  • Product name
    Anti-BBS2 antibody
    See all BBS2 primary antibodies
  • Description
    Mouse polyclonal to BBS2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Full length BBS2 protein (Human)

  • Positive control
    • BBS2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

Target

  • Relevance
    Defects in BBS2 are the cause of Bardet-Biedl syndrome type 2. Bardet-Biedl syndrome is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • Bardet Biedl syndrome 2 antibody
      • Bardet Biedl syndrome 2 protein antibody
      • BBS antibody
      • MGC20703 antibody

    Anti-BBS2 antibody images

    • Western blot - BBS2 antibody (ab68223)
      Western blot - BBS2 antibody (ab68223)
      All lanes : Anti-BBS2 antibody (ab68223) at 1/500 dilution

      Lane 1 : BBS2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 80 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References for Anti-BBS2 antibody (ab68223)

    ab68223 has not yet been referenced specifically in any publications.

    Publishing research using ab68223? Please let us know so that we can cite the reference in this datasheet.

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    1 Abreview or Q&A

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    Western blot abreview for Anti-BBS2 antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (kidney cells)
    Loading amount
    20 µg
    Specification
    kidney cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Aug 31 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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