Anti-BBS4 antibody [1292CT845.130.218] (ab188364)

Overview

  • Product name
    Anti-BBS4 antibody [1292CT845.130.218]
    See all BBS4 primary antibodies
  • Description
    Mouse monoclonal [1292CT845.130.218] to BBS4
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BBS4 aa 1-240. The exact sequence is proprietary.
    Database link: Q96RK4

  • Positive control
    • HeLa, U251, mouse heart and kidney, rat heart and kidney lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    1292CT845.130.218
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • Function
    May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
  • Tissue specificity
    Ubiquitously expressed. The highest level of expression is found in the kidney.
  • Involvement in disease
    Defects in BBS4 are the cause of Bardet-Biedl syndrome type 4 (BBS4) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect.
  • Sequence similarities
    Belongs to the BBS4 family.
    Contains 10 TPR repeats.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton. Cell projection > cilium membrane. Cytoplasm. Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bardet Biedl syndrome 4 protein antibody
    • Bardet-Biedl syndrome 4 protein antibody
    • Bbs4 antibody
    • BBS4_HUMAN antibody

Images

  • All lanes : Anti-BBS4 antibody [1292CT845.130.218] (ab188364) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : U251 lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat kidney lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG H&L(HRP) at 1/3000 dilution

References

ab188364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (adipocyte- F442A cell)
Permeabilization
Yes - 0.1% Triton (x100)
Specification
adipocyte- F442A cell
Blocking step
INGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Mariana Anosov

Verified customer

Submitted Apr 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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