Overview

  • Product name
  • Description
    Rabbit polyclonal to BCA1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hBCA-1 (human B-Cell Attracting Chemokine-1)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA:To detect hBCA-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBCA-1.

    Western Blot:To detect hBCA-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBCA-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • Function
      Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
    • Tissue specificity
      Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
    • Sequence similarities
      Belongs to the intercrine alpha (chemokine CxC) family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANGIE antibody
      • ANGIE2 antibody
      • B cell attracting chemokine 1 antibody
      • B cell-attracting chemokine 1 antibody
      • B lymphocyte chemoattractant antibody
      • B-cell chemoattractant antibody
      • B-cell-attracting chemokine 1 antibody
      • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) antibody
      • BCA-1 antibody
      • BLC antibody
      • BLR1L antibody
      • C-X-C motif chemokine 13 antibody
      • Chemokine (C-X-C motif) ligand 13 antibody
      • Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) antibody
      • Chemokine, CXC motif, ligand 13 antibody
      • CXC chemokine BLC antibody
      • CXCL13 antibody
      • CXL13_HUMAN antibody
      • SCYB13 antibody
      • Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant) antibody
      • Small inducible cytokine B13 antibody
      • Small inducible cytokine subfamily B, member 13 antibody
      • Small-inducible cytokine B13 antibody
      see all

    References for Anti-BCA1 antibody (ab9975)

    ab9975 has not yet been referenced specifically in any publications.

    Product Wall

    To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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