Overview

  • Product name
    Anti-BCA225 antibody [CU18]
  • Description
    Mouse monoclonal [CU18] to BCA225
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BCA 225 protein secreted by the T47D (Clone 11) human breast carcinoma cellline.

  • Positive control
    • Breast carcinoma (human).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    CU18
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    Immunohistochemistry: 1/40.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    Antigen retrieval is not essential, although may be beneficial.

    Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus.
    Similar staining patterns are observed in lung, ovarian, and endometrialcancers.
    Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.
  • Target

    • Relevance
      BCA225 is a 225 kDa glycoprotein secreted by the T47D breast carcinoma cell line.
    • Alternative names
      • BRST1 antibody

    References

    This product has been referenced in:
    • Nishikata T  et al. Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells. Anticancer Res 33:2867-73 (2013). Read more (PubMed: 23780972) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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