The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesImmunohistochemistry: 1/40.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
Antigen retrieval is not essential, although may be beneficial.
Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus.
Similar staining patterns are observed in lung, ovarian, and endometrialcancers.
Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.
RelevanceBCA225 is a 225 kDa glycoprotein secreted by the T47D breast carcinoma cell line.
References for Anti-BCA225 antibody [CU18] (ab3360)
This product has been referenced in:
Nishikata T et al. Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells. Anticancer Res33:2867-73 (2013).
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