Overview

  • Product name
  • Description
    Goat polyclonal to BCAR1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human BCAR1 aa 143-155 (internal sequence) (Cysteine residue). (NP_001164185.1; NP_001164186.1; NP_001164187.1; NP_001164188.1; NP_001164189.1; NP_055382.2; NP_001164190.1; NP_001164191.1; NP_001164192.1).
    Sequence:

    KQTSTFSKQTPHH


    Database link: P56945

  • Positive control
    • MCF7 cell lysate; Human Breast cancer lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab178524 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 93 kDa.

Target

  • Function
    Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • Tissue specificity
    Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • Sequence similarities
    Belongs to the CAS family.
    Contains 1 SH3 domain.
  • Domain
    Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • Post-translational
    modifications
    PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • Cellular localization
    Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Images

  • All lanes : Anti-BCAR1 antibody (ab178524) at 1 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : Human Breast cancer lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 93 kDa

References

ab178524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab178524.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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