Overview

  • Product nameAnti-BCAR1 antibody [CAS-14]
    See all BCAR1 primary antibodies
  • Description
    Mouse monoclonal [CAS-14] to BCAR1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length Human BCAR1

  • Positive control
    • MCF-7 cells, HUVEC cells. Tonsil or breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, boiling tissue sections in 10mM citrate buffer, pH 6.0.
    IP: Use at 2µg/mg of lysate. Denatured verified. Use Protein G.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDocking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
    • Tissue specificityWidely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
    • Sequence similaritiesBelongs to the CAS family.
      Contains 1 SH3 domain.
    • DomainContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
      A serine-rich region promotes activation of the serum response element (SRE).
      The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
    • Post-translational
      modifications
      PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
      Dephosphorylated by PTPN14 at Tyr-128.
    • Cellular localizationCell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • BCAR 1 antibody
      • Bcar1 antibody
      • BCAR1_HUMAN antibody
      • Breast cancer anti estrogen resistance 1 antibody
      • Breast cancer anti estrogen resistance 1 protein antibody
      • Breast cancer anti-estrogen resistance protein 1 antibody
      • CAS antibody
      • Cas scaffolding protein family member 1 antibody
      • CAS1 antibody
      • Cass1 antibody
      • Crk associated substrate antibody
      • Crk associated substrate p130Cas antibody
      • CRK-associated substrate antibody
      • CRKAS antibody
      • FLJ12176 antibody
      • FLJ45059 antibody
      • p130cas antibody
      see all

    References for Anti-BCAR1 antibody [CAS-14] (ab80619)

    ab80619 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"