Recombinant Anti-BCAR1 antibody [EPR1850(2)] (ab108320)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1850(2)] to BCAR1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BCAR1 antibody [EPR1850(2)]
See all BCAR1 primary antibodies -
Description
Rabbit monoclonal [EPR1850(2)] to BCAR1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, T47-D, A431, C6, Raw264.7, PC12, and NIH 3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1850(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 93 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 93 kDa. |
Target
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Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
Tissue specificity
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
Sequence similarities
Belongs to the CAS family.
Contains 1 SH3 domain. -
Domain
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
Post-translational
modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
Cellular localization
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
- Unigene: 479747 Human
see all -
Alternative names
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
Images
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All lanes : Anti-BCAR1 antibody [EPR1850(2)] (ab108320) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108320 has been referenced in 1 publication.
- Holle AW et al. High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integr Biol (Camb) 8:1049-1058 (2016). PubMed: 27723854