Anti-BCAR1 (phospho Y410) antibody (ab55263)

Overview

  • Product name
    Anti-BCAR1 (phospho Y410) antibody
    See all BCAR1 primary antibodies
  • Description
    Rabbit polyclonal to BCAR1 (phospho Y410)
  • Specificity
    ab55263 detects endogenous levels of BCAR1 only when phosphorylated at tyrosine 410.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from BCAR1 around the phosphorylation site of tyrosine 410 (G-V-YP-A-V).

  • Positive control
    • human breast carcinoma tissue, extracts from NIH/3T3 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • Tissue specificity
    Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • Sequence similarities
    Belongs to the CAS family.
    Contains 1 SH3 domain.
  • Domain
    Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • Post-translational
    modifications
    PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • Cellular localization
    Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Images

  • All lanes : Anti-BCAR1 (phospho Y410) antibody (ab55263) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells.
    Lane 2 : extracts from NIH/3T3 cells, incubated with the immunising phosphopeptide.


    Predicted band size : 94 kDa
    Observed band size : 94 kDa
  • Immunohistochemical analysis of BCAR1 (phospho Y410) expression in paraffin embedded human breast carcinoma tissue, using 1/50 ab55263. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

References

ab55263 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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