Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699)


  • Product nameAnti-BCAR1 (phospho Y410) antibody [EPR1860]
    See all BCAR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1860] to BCAR1 (phospho Y410)
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Tyrosine 410 of Human BCAR1.

  • Positive control
    • HeLa cell lysate treated with pervanadate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: 1/50.
    WB: 1/2500 - 1/5000. Predicted molecular weight: 93 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDocking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
    • Tissue specificityWidely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
    • Sequence similaritiesBelongs to the CAS family.
      Contains 1 SH3 domain.
    • DomainContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
      A serine-rich region promotes activation of the serum response element (SRE).
      The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
    • Post-translational
      PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
      Dephosphorylated by PTPN14 at Tyr-128.
    • Cellular localizationCell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • BCAR 1 antibody
      • Bcar1 antibody
      • BCAR1_HUMAN antibody
      • Breast cancer anti estrogen resistance 1 antibody
      • Breast cancer anti estrogen resistance 1 protein antibody
      • Breast cancer anti-estrogen resistance protein 1 antibody
      • CAS antibody
      • Cas scaffolding protein family member 1 antibody
      • CAS1 antibody
      • Cass1 antibody
      • Crk associated substrate antibody
      • Crk associated substrate p130Cas antibody
      • CRK-associated substrate antibody
      • CRKAS antibody
      • FLJ12176 antibody
      • FLJ45059 antibody
      • p130cas antibody
      see all

    Anti-BCAR1 (phospho Y410) antibody [EPR1860] images

    • All lanes : Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699) at 1/2500 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 93 kDa

    References for Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699)

    ab92699 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92699.
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