Recombinant
RabMAb

Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699)

Overview

  • Product name
    Anti-BCAR1 (phospho Y410) antibody [EPR1860]
    See all BCAR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1860] to BCAR1 (phospho Y410)
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Tyrosine 410 of Human BCAR1.

  • Positive control
    • HeLa cell lysate treated with pervanadate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/50.
    WB: 1/2500 - 1/5000. Predicted molecular weight: 93 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
    • Tissue specificity
      Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
    • Sequence similarities
      Belongs to the CAS family.
      Contains 1 SH3 domain.
    • Domain
      Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
      A serine-rich region promotes activation of the serum response element (SRE).
      The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
    • Post-translational
      modifications
      PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
      Dephosphorylated by PTPN14 at Tyr-128.
    • Cellular localization
      Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • BCAR 1 antibody
      • Bcar1 antibody
      • BCAR1_HUMAN antibody
      • Breast cancer anti estrogen resistance 1 antibody
      • Breast cancer anti estrogen resistance 1 protein antibody
      • Breast cancer anti-estrogen resistance protein 1 antibody
      • CAS antibody
      • Cas scaffolding protein family member 1 antibody
      • CAS1 antibody
      • Cass1 antibody
      • Crk associated substrate antibody
      • Crk associated substrate p130Cas antibody
      • CRK-associated substrate antibody
      • CRKAS antibody
      • FLJ12176 antibody
      • FLJ45059 antibody
      • p130cas antibody
      see all

    Images

    • All lanes : Anti-BCAR1 (phospho Y410) antibody [EPR1860] (ab92699) at 1/2500 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 93 kDa

    References

    ab92699 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92699.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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