SpecificityThis antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.
Synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src binding domain of rat BCAR1. This rat sequence is the same as Tyr-664 in human BCAR1 and Tyr-664 in mouse BCAR1. This site is also highly conserved in Cas-L (HEF1, Nedd9).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Purification notesThis antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 130 kDa.
Use at an assay dependent concentration. PubMed: 22084245
FunctionDocking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
Tissue specificityWidely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
Sequence similaritiesBelongs to the CAS family. Contains 1 SH3 domain.
DomainContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9. A serine-rich region promotes activation of the serum response element (SRE). The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
Post-translational modificationsPTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128.
Cellular localizationCell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
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Anti-BCAR1 (phospho Y762) antibody images
Western blot - BCAR1 (phospho Y762) antibody (ab41827)
Predicted band size : 130 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BCAR1 (phospho Y762) antibody (ab41827)Image from Zheng Y et al, J Biol Chem. 2012 Jan 2;287(1):148-58. Epub 2011 Nov 14, Fig 4. DOI 10.1074/jbc.M111.298117 January 2, 2012 The Journal of Biological Chemistry, 287, 148-158.
ab41827 staining BCAR1 in stable PC3 cells by Immunocytochemistry/ Immunofluorescence. Cells were counterstained with DAPI (blue).
References for Anti-BCAR1 (phospho Y762) antibody (ab41827)
This product has been referenced in:
Zheng Y et al. Protein-tyrosine Kinase 6 Promotes Peripheral Adhesion Complex Formation and Cell Migration by Phosphorylating p130 CRK-associated Substrate. J Biol Chem287:148-58 (2012).
Read more (PubMed: 22084245) »