The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Tissue specificityUbiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.