Overview

  • Product name
  • Description
    Rabbit polyclonal to BCAS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 19-204 of Human BCAS2

  • Positive control
    • NT2D1 cells; IMR32 cells; MCF-7 cell lysate; HeLa cells; J5 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab151293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in mRNA splicing.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SPF27 family.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bcas2 antibody
    • Breast carcinoma amplified sequence 2 antibody
    • Breast carcinoma-amplified sequence 2 antibody
    • DAM1 antibody
    • DNA amplified in mammary carcinoma 1 protein antibody
    • MGC7712 antibody
    • Pre mRNA splicing factor SPF27 antibody
    • Pre-mRNA-splicing factor spf27 antibody
    • Snt309 antibody
    • SPF27 antibody
    • SPF27_HUMAN antibody
    • Spliceosome associated protein amplified in breast cancer antibody
    • Spliceosome associated protein SPF 27 antibody
    • Spliceosome-associated protein SPF 27 antibody
    see all

Images

  • Anti-BCAS2 antibody (ab151293) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded J5 xenograft labeling BCAS2 with ab151293 antibody at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling BCAS2 with ab151293 antibody at 1/200 dilution. Bottom figure represents cells costained with Hoechst 33342.

References

ab151293 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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