Overview

  • Product name
  • Description
    Rabbit polyclonal to BCAS2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 175 and the C-terminus (residue 225) of human BCAS2 (NP_005863.1)

  • Positive control
    • Whole cell lysate from MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in mRNA splicing.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SPF27 family.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bcas2 antibody
    • Breast carcinoma amplified sequence 2 antibody
    • Breast carcinoma-amplified sequence 2 antibody
    • DAM1 antibody
    • DNA amplified in mammary carcinoma 1 protein antibody
    • MGC7712 antibody
    • Pre mRNA splicing factor SPF27 antibody
    • Pre-mRNA-splicing factor spf27 antibody
    • Snt309 antibody
    • SPF27 antibody
    • SPF27_HUMAN antibody
    • Spliceosome associated protein amplified in breast cancer antibody
    • Spliceosome associated protein SPF 27 antibody
    • Spliceosome-associated protein SPF 27 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer (left) and mouse squamous cell carcinoma (right) tissues labelling BCAS2 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Detection of Human BCAS2 by Immunoprecipitation in Whole cell lysate from MCF7 cells (1 mg for IP, 20% of IP loaded) using ab71161 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References

ab71161 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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