Overview

  • Product name
  • Description
    Rabbit polyclonal to BCAS2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 175 and the C-terminus (residue 225) of human BCAS2 (NP_005863.1)

  • Positive control
    • Whole cell lysate from MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in mRNA splicing.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SPF27 family.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bcas2 antibody
    • Breast carcinoma amplified sequence 2 antibody
    • Breast carcinoma-amplified sequence 2 antibody
    • DAM1 antibody
    • DNA amplified in mammary carcinoma 1 protein antibody
    • MGC7712 antibody
    • Pre mRNA splicing factor SPF27 antibody
    • Pre-mRNA-splicing factor spf27 antibody
    • Snt309 antibody
    • SPF27 antibody
    • SPF27_HUMAN antibody
    • Spliceosome associated protein amplified in breast cancer antibody
    • Spliceosome associated protein SPF 27 antibody
    • Spliceosome-associated protein SPF 27 antibody
    see all

Anti-BCAS2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer (left) and mouse squamous cell carcinoma (right) tissues labelling BCAS2 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Detection of Human BCAS2 by Immunoprecipitation in Whole cell lysate from MCF7 cells (1 mg for IP, 20% of IP loaded) using ab71161 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References for Anti-BCAS2 antibody (ab71161)

ab71161 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71161.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up