Overview

  • Product nameAnti-BCAS3 antibody [2066C2a]
    See all BCAS3 primary antibodies
  • Description
    Mouse monoclonal [2066C2a] to BCAS3
  • Tested applicationsSuitable for: WB, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human BCAS3 fragment

  • Epitopeab50973 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • Positive control
    • HeLa cells, NIH3T3 whole cell lysate and A2058 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa).
ICC 1/10.
Dot blot Use at an assay dependent dilution.

Target

  • Tissue specificityExpressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
  • Involvement in diseaseNote=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
  • Sequence similaritiesBelongs to the WD repeat BCAS3 family.
    Contains 3 WD repeats.
  • Developmental stageFetal.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500019F07Rik antibody
    • 2610028P08Rik antibody
    • AU021018 antibody
    • BC028339 antibody
    • BCAS3 antibody
    • BCAS3_HUMAN antibody
    • Breast carcinoma-amplified sequence 3 antibody
    • FLJ20128 antibody
    • GAOB1 antibody
    • K20D4 antibody
    • MAAB antibody
    • Maab1 protein antibody
    • MGC4973 antibody
    • rudhira antibody
    see all

Anti-BCAS3 antibody [2066C2a] images

  • Anti-BCAS3 antibody [2066C2a] (ab50973) at 1/100 dilution + A2058 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)
  • ab50973 at 1/10 dilution staining human BCAS3 in HeLa cells by Immunocytochemistry. Goat Anti-mouse IgG at 1/200 was used as a secondary antibody.
  • Anti-BCAS3 antibody [2066C2a] (ab50973) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)

References for Anti-BCAS3 antibody [2066C2a] (ab50973)

ab50973 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"