The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Tissue specificityExpressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
Involvement in diseaseNote=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
Sequence similaritiesBelongs to the WD repeat BCAS3 family. Contains 3 WD repeats.
Immunoprecipitation - Breast carcinoma amplified sequence 3 antibody (ab71162)
Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1: ab71162 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
References for Anti-BCAS3 antibody (ab71162)
has not yet been referenced specifically in any publications.
Publishing research using ab71162? Please let us know so that we can cite the reference in this datasheet.
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