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Our Abpromise guarantee covers the use of ab125209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. The detection limit for BCAT 1 is approximately 0.1 ng/lane under non-reducing and reducing conditions.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|