The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. The detection limit for BCAT2 is approximately 1ng/lane under non-reducing and reducing conditions.
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionCatalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
Sequence similaritiesBelongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.