Overview

  • Product nameAnti-BCAT2 antibody
    See all BCAT2 primary antibodies
  • Description
    Rabbit polyclonal to BCAT2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C terminal amino acids of Human BCAT2.

  • Positive control
    • Human Colon cancer tissue; A431, SW620, Smmc, Jurkat and CEM whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab125222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. The detection limit for BCAT2 is approximately 1ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localizationCytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCAM antibody
    • BCAT 2 antibody
    • BCAT(m) antibody
    • BCAT2 antibody
    • BCAT2_HUMAN antibody
    • BCATM antibody
    • BCT2 antibody
    • Branched chain aminotransferase 2, mitochondrial antibody
    • Branched-chain-amino-acid aminotransferase antibody
    • ECA40 antibody
    • mitochondrial antibody
    • Placental protein 18 antibody
    • PP18 antibody
    see all

Anti-BCAT2 antibody images

  • ab125222, at 1µg/ml, staining BCAT2 in Paraffin-embedded Human colon cancer tissue by Immunohistochemistry.
  • All lanes : Anti-BCAT2 antibody (ab125222) at 1 µg/ml

    Lane 1 : A431 whole cell lysate
    Lane 2 : SW620 whole cell lysate
    Lane 3 : Smmc whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : CEM whole cell lysate


    Predicted band size : 44 kDa

References for Anti-BCAT2 antibody (ab125222)

ab125222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"