Anti-BCCIP antibody (ab97577)
Key features and details
- Rabbit polyclonal to BCCIP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCCIP antibody
See all BCCIP primary antibodies -
Description
Rabbit polyclonal to BCCIP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 181-243 of Human BCCIP (Q9P287)
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Positive control
- A431, Hela, 293T or HepG2 cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/200.
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Notes |
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WB
1/1000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/200. |
Target
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Function
May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ). -
Tissue specificity
Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen. -
Sequence similarities
Belongs to the BCP1 family. -
Developmental stage
Isoform 1 is expressed throughout the cell cycle. Isoform 2 is expressed following mitosis and peaks in the G1/S phase of the cell cycle. -
Cellular localization
Nucleus. Colocalizes with BRCA2 in discrete nuclear foci. - Information by UniProt
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Database links
- Entrez Gene: 56647 Human
- Omim: 611883 Human
- SwissProt: Q9P287 Human
- Unigene: 370292 Human
- Unigene: 715543 Human
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Alternative names
- bccip antibody
- BCCIP_HUMAN antibody
- BCCIPalpha antibody
see all
Images
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All lanes : Anti-BCCIP antibody (ab97577) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS Page -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97577 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab97577 has not yet been referenced specifically in any publications.