Overview

  • Product name
    BCIP toluidine salt
  • Description
    Histochemical alkaline phosphatase substrate.
  • Alternative names
    • 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
    • BCIP 4-toluidine salt
    • BCIP p-toluidine salt
    • X-phosphate p-toluidine salt
  • Biological description

    Chromogenic substrate for alkaline phosphatase. Often used in combination with nitro blue tetrazolium chloride (NBT) to amplify the sensitivity of staining.

  • Purity
    > 99%

Properties

  • Molecular weight
    433.62
  • Chemical structure
    Chemical Structure
  • Molecular formula
    C8H6BrClNO4P.C7H9N
  • CAS Number
    6578-06-9
  • Storage instructions
    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:
  • Lee SW  et al. Enhanced biomolecular detection based on localized surface plasmon resonance (LSPR) using enzyme-precipitation reaction. J Nanosci Nanotechnol 10:3246-9 (2010). Read more (PubMed: 20358932) »
  • Meltzer JC  et al. Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. J Histochem Cytochem 45:599-610 (1997). Read more (PubMed: 9111238) »
  • Okamoto Y  et al. Detection of interleukin 6-producing cells among various organs in normal mice with an improved enzyme-linked immunospot (ELISPOT) assay. Endocr J 44:349-55 (1997). Read more (PubMed: 9279509) »
  • Leary JJ  et al. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci U S A 80:4045-9 (1983). Read more (PubMed: 6191328) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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