Overview

  • Product name
    Anti-BCKDH kinase antibody
    See all BCKDH kinase primary antibodies
  • Description
    Rabbit polyclonal to BCKDH kinase
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 46-287 of Human BCKDH kinase (AAH07363).

  • Positive control
    • 293T and Mouse brain whole cell lysates; Human gastric carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)

Target

  • Function
    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Images

  • Anti-BCKDH kinase antibody (ab111716) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 46 kDa
  • Anti-BCKDH kinase antibody (ab111716) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 46 kDa
  • ab111716 at 1/500 dilution staining BCKDH kinase in Formalin-fixed, paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.

References

This product has been referenced in:
  • Bravo-Alonso I  et al. Dataset reporting BCKDK interference in a BCAA-catabolism restricted environment. Data Brief 7:755-9 (2016). WB . Read more (PubMed: 27054191) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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