Overview

  • Product name
    Anti-BCKDH kinase antibody
    See all BCKDH kinase primary antibodies
  • Description
    Rabbit polyclonal to BCKDH kinase
  • Specificity
    ab65027 detects endogenous levels of total BCKDH kinase protein.
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human BCKDH kinase

  • Positive control
    • 293 and Jurkat cell extracts and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • Function
    Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Images

  • ab65027 at 1/500 - 1/1000 dilution staining BCKDH kinase in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-BCKDH kinase antibody (ab65027) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : Jurkat cell extract with immunising peptide


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

References

ab65027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab65027.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up