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Rabbit polyclonal to BCKDHA
Recombinant fragment, corresponding to a region within amino acids 252-432 of Human BCKDHA (UniProt P12694).
HepG2 whole cell lysate; HeLa cells; Human Colon cancer tissue; A549
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/500.
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Involvement in disease
Maple syrup urine disease 1A
Belongs to the BCKDHA family.
Information by UniProt
Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
Western blot - Anti-BCKDHA antibody (ab126173)
Anti-BCKDHA antibody (ab126173) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size : 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BCKDHA antibody (ab126173)
ab126173, at 1/500 dilution, staining BCKDHA in methanol-fixed HeLa cells by Immunofluorescence. The lower panel is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody (ab126173)
ab126173, at 1/250 dilution, staining BCKDHA in paraffin-embedded Human Colon cancer tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"