Overview

  • Product name
  • Description
    Rabbit polyclonal to BCKDHA
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 252-432 of Human BCKDHA (UniProt P12694).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; Human Colon cancer tissue; A549

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab126173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Maple syrup urine disease 1A
  • Sequence similarities
    Belongs to the BCKDHA family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
    • FLJ45695 antibody
    • OVD1A antibody
    • 2 oxoisovalerate dehydrogenase (lipoamide) antibody
    • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
    • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
    • BCKDE1A antibody
    • BCKDH E1 alpha antibody
    • BCKDH E1-alpha antibody
    • BCKDHA antibody
    • Branched chain alpha keto acid dehydrogenase E1 component alpha chain antibody
    • Branched chain keto acid dehydrogenase E1 alpha polypeptide antibody
    • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) antibody
    • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody
    • MSU antibody
    • MSUD1 antibody
    • ODBA_HUMAN antibody
    see all

Images

  • Anti-BCKDHA antibody (ab126173) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • ab126173, at 1/500 dilution, staining BCKDHA in methanol-fixed HeLa cells by Immunofluorescence. The lower panel is costained with Hoechst 33342.
  • ab126173, at 1/250 dilution, staining BCKDHA in paraffin-embedded Human Colon cancer tissue by Immunohistochemistry.

References

ab126173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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