Overview

  • Product name
  • Description
    Mouse polyclonal to BCKDHA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Caenorhabditis elegans
  • Immunogen

    Full length protein, corresponding to amino acids 1-445 of Human BCKDHA

  • Positive control
    • Transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab68094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Involvement in disease
      Maple syrup urine disease 1A
    • Sequence similarities
      Belongs to the BCKDHA family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
      • FLJ45695 antibody
      • OVD1A antibody
      • 2 oxoisovalerate dehydrogenase (lipoamide) antibody
      • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • BCKDE1A antibody
      • BCKDH E1 alpha antibody
      • BCKDH E1-alpha antibody
      • BCKDHA antibody
      • Branched chain alpha keto acid dehydrogenase E1 component alpha chain antibody
      • Branched chain keto acid dehydrogenase E1 alpha polypeptide antibody
      • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) antibody
      • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody
      • MSU antibody
      • MSUD1 antibody
      • ODBA_HUMAN antibody
      see all

    Anti-BCKDHA antibody images

    • All lanes : Anti-BCKDHA antibody (ab68094) at 1/500 dilution

      Lane 1 : BCKDHA transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 50 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References for Anti-BCKDHA antibody (ab68094)

    ab68094 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Caenorhabditis elegans Tissue lysate - whole (Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu)
    Specification
    Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Julianne Martell

    Verified customer

    Submitted Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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