Overview

  • Product nameAnti-BCKDHA antibody
    See all BCKDHA primary antibodies
  • Description
    Rabbit polyclonal to BCKDHA
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region within N terminal amino acids 72 - 121 (VISGIPIYRV MDRQGQIINP SEDPHLPKEK VLKLYKSMTL LNTMDRILYE) of Human BCKDHA (NP_000700).

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab90691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with BCKDHA peptide (ab134676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-BCKDHA antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling BCKDHA with ab90691 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I and II cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - BCKDHA. Right - Merge.
  • Anti-BCKDHA antibody (ab90691) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Gel concentration: 12%

References for Anti-BCKDHA antibody (ab90691)

This product has been referenced in:
  • Kitsy A  et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . Read more (PubMed: 25050624) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"