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Synthetic peptide corresponding to Human Bcl-2.
Database link: P10415
Store at -20°C for year
Our Abpromise guarantee covers the use of ab59348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 26 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab59348, at a 1/50 dilution, staining human Bcl-2 in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
ELISA analysis of primary cultured rat cortical neurons, detecting Bcl-2 using ab59348.
Cells were treated with (Tat) or without Tat (control) and either Estradiol, Genistein or Daidzein. Lysates were prepared after 24 hours. A 96-well plate was coated using carbonate coating buffer. 20 µg cell lysate was added to wells and incubated overnight at 4°C. Plates were blocked with 1% BSA for 2 hr at room temperature. Primary antibody (1/5000) was added to sample wells before incubating overnight at 4°C. A goat anti-rabbit ALP-conjugated IgG was used as secondary antibody and phosphatase substrate mixture was added. Absorbance was read at 650nm.
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Bcl-2 with ab59348 in the absence (left image) and presence of the immunizing peptide (right image).