Our Abpromise guarantee covers the use of ab117115 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BCL2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: THP-1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab117115 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab117115 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab117115 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of dog breast cancer tissue sections labeling Bcl-2 with ab117115 at 1/50 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using EDTA. An undiluted polyclonal mouse biotinylated secondary antibody was used.