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Does not react withRat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bcl-2 aa 50-150.
Database link: P10415
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
This product is available conjugated to DyLight® 488 - see ab139926.
A trial size is available for this product.
Our Abpromise guarantee covers the use of ab32124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||Use at an assay dependent concentration.
We do not recommend rat and mouse samples with IHC.
Immunofluorescent staining of MCF-7 cells using anti-Bcl-2 RabMAB(green) diluted 1/100 and counter stain with anti-tubulin RabMAB(red) shows staining in the cytoplasm.
Immunohistochemical analysis of Human salivary glands taken from patients with primary Sjögren's syndrome, staining Bcl-2 with ab32124.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked using 2% BSA, 10% normal serum and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100) for one hour at room temperature. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.
N.B. Panels B and D are higher magnifications of panels A and C, respectively.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"