Recombinant
RabMAb

Anti-bcl-6 antibody [EPR11410-43] (ab172610)

Overview

  • Product name
    Anti-bcl-6 antibody [EPR11410-43]
    See all bcl-6 primary antibodies
  • Description
    Rabbit monoclonal [EPR11410-43] to bcl-6
  • Specificity
    We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P41182

  • Positive control
    • Ramos, Daudi and Raji cell lysates; Human lymph node, tonsil and B cell lymphoma tissues; Ramos cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab172610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • B Cell Lymphoma 6 Protein antibody
      • B-cell CLL/lymphoma 6 antibody
      • B-cell lymphoma 5 protein antibody
      • B-cell lymphoma 6 protein transcript antibody
      • BCL5 antibody
      • BCL6A antibody
      • cys-his2 zinc finger transcription factor antibody
      • LAZ3 antibody
      • lymphoma-associated zinc finger gene on chromosome 3 antibody
      • Protein LAZ-3 antibody
      • ZBTB27 antibody
      • Zinc finger and BTB domain-containing protein 27 antibody
      • Zinc Finger Protein 51 antibody
      • zinc finger transcription factor BCL6S antibody
      • ZNF51 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution.

    • All lanes : Anti-bcl-6 antibody [EPR11410-43] (ab172610) at 1000

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 78 kDa
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution.

    References

    This product has been referenced in:
    • Song Y  et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC ; Human . Read more (PubMed: 27076925) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 08 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 19 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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