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Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P41182
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab172610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution.
ab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"