Overview

  • Product nameAnti-Bcl x antibodySee all Bcl x primary antibodies ...
  • Description
    Rabbit polyclonal to Bcl x
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Cat, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 178 of Human Bcl x (NP_612815).

  • Positive control
    • A549, HepG2, HCT116, NIH 3T3 cell lysates; SW480 xenograft This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa and HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab98143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceBcl x is a member of the Bcl 2 family of proteins, which function as regulators of apoptosis. It may provide neuroprotection against ischemic brian injury. Bcl x has two isoforms, Bcl xl (long) a 241 amino acid protein and Bcl xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl2 family. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
  • Cellular localizationMitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Note: Mitochondrial membranes and perinuclear envelope. Cytoskeleton
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Bcl 2 like 1 antibody
    • Bcl 2 like 1 protein antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl2 like1 antibody
    • BCL2-related gene antibody
    • BCL2-related protein, long isoform, included; BCLXL, included antibody
    • BCL2-related protein, short isoform, included; BCLXS, included antibody
    • BCL2L antibody
    • BCL2L1 antibody
    • Bclx antibody
    • DKFZp781P2092 antibody
    • MGC113803 antibody
    • MGC99998 antibody
    see all

Anti-Bcl x antibody images

  • ICC/IF image of ab98143 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98143 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) hepG2 cells at 5ug/ml.

  • Anti-Bcl x antibody (ab98143) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 26 kDa
    12% SDS PAGE
  • Anti-Bcl x antibody (ab98143) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 26 kDa
    12% SDS PAGE
  • Anti-Bcl x antibody (ab98143) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 26 kDa
    12% SDS PAGE
  • Immunohistochemical analysis of Bcl x in paraffin embedded SW480 xenograft, using ab98143 at a 1/500 dilution.

References for Anti-Bcl x antibody (ab98143)

ab98143 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (prostate)
Specification prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"