• Product nameAnti-Bcl-XL antibody [2H12] (Biotin)
    See all Bcl-XL primary antibodies
  • Description
    Mouse monoclonal [2H12] to Bcl-XL (Biotin)
  • ConjugationBiotin
  • Specificityab79460 reacts with both isoforms of BCL-XL.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-XL aa 3-14.


    Database link: Q07817

  • Positive control
    • Hodgkin’s lymphoma tissue.



Our Abpromise guarantee covers the use of ab79460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. WB: Use at an assay dependent dilution.

Flow Cyt Use at an assay dependent concentration.

Use at an assay dependent dilution.

ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.


  • FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

References for Anti-Bcl-XL antibody [2H12] (Biotin) (ab79460)

This product has been referenced in:
  • Hsu YT & Youle RJ Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 272:13829-34 (1997). Read more (PubMed: 9153240) »
  • Hsu YT  et al. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A 94:3668-72 (1997). Read more (PubMed: 9108035) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products. Neither of these two antibodies has been tested in rabbit, though due to sequence homology the cross-reactivity with this species is expected. It is important to mention that both ab54396...

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