Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)

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Overview

  • Product nameAnti-Bcl-XL antibody [7B2.5] (FITC)
    See all Bcl-XL primary antibodies
  • Description
    Mouse monoclonal [7B2.5] to Bcl-XL (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Bcl-XL.
    Database link: Q07817

Applications

Our Abpromise guarantee covers the use of ab26148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab91539-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

Target

  • FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody [7B2.5] (FITC) images

  • Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)
    Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)

    Flow Cytometry analysis of T cell leukemia cell line Jurkat intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (ab26148) 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG3-FITC.

  • Flow Cytometry - Anti-Bcl-XL [7B2.5] antibody (FITC) (ab26148)
    Flow Cytometry - Anti-Bcl-XL [7B2.5] antibody (FITC) (ab26148)
    Overlay histogram showing DU145 cells stained with ab26148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26148, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

References for Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)

ab26148 has not yet been referenced specifically in any publications.

Publishing research using ab26148? Please let us know so that we can cite the reference in this datasheet.

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2 Abreviews or Q&A

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Votes: Highest First

I was wondering if you can provide me with an estimate as to how strong the intensity of your Bcl-xL-FITC (ab26148) antibody is when compared to unstained cells, when used in a flow cytometry setting (ie: would it be upwards of 2-log shift in high-bcl...

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Thank you for your enquiry. I am looking into your question about the intensity of the antibody relative to unstained cells, and will get back to you once I have further information. As to your other question, interestingly enough I was discussin...

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I am interested in purchasing your anti-Bcl-xL monoclonal antibody for measurement of Bcl-xL levels in murine primary B cells by FACS. My FACS color options for my other stains is quite broad, so it is not an issue to me whether I purchase the anti-Bc...

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Thank you for your enquiry. I have not heard of a difference between, for example, PE or FITC conjugated antibodies for intracellular staining in FACS. I consulted with several colleagues as well as searched the web and other antibody company's web...

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