Overview

  • Product name
    Anti-Bcl-XL antibody [7B2.5] (FITC)
    See all Bcl-XL primary antibodies
  • Description
    Mouse monoclonal [7B2.5] to Bcl-XL (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Bcl-XL.
    Database link: Q07817

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Applications

Our Abpromise guarantee covers the use of ab26148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab91539 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

Target

  • Function
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificity
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localization
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Images

  • Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)
    Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)

    Flow Cytometry analysis of T cell leukemia cell line Jurkat intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (ab26148) 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG3-FITC.

  • Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)
    Flow Cytometry - Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)
    Overlay histogram showing DU145 cells stained with ab26148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26148, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Chong HZ  et al. In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis. BMC Complement Altern Med 12:134 (2012). Flow Cyt . Read more (PubMed: 22909149) »
  • Chong HZ  et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012). Flow Cyt . Read more (PubMed: 22471785) »

See all 2 Publications for this product

Publishing research using ab26148? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

I was wondering if you can provide me with an estimate as to how strong the intensity of your Bcl-xL-FITC (ab26148) antibody is when compared to unstained cells, when used in a flow cytometry setting (ie: would it be upwards of 2-log shift in high-bcl...

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Thank you for your enquiry. I am looking into your question about the intensity of the antibody relative to unstained cells, and will get back to you once I have further information. As to your other question, interestingly enough I was discussin...

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I am interested in purchasing your anti-Bcl-xL monoclonal antibody for measurement of Bcl-xL levels in murine primary B cells by FACS. My FACS color options for my other stains is quite broad, so it is not an issue to me whether I purchase the anti-Bc...

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Thank you for your enquiry. I have not heard of a difference between, for example, PE or FITC conjugated antibodies for intracellular staining in FACS. I consulted with several colleagues as well as searched the web and other antibody company's web...

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