Anti-Bcl-XL antibody [7B2.5] (Phycoerythrin) (ab26035)

Overview

  • Product nameAnti-Bcl-XL antibody [7B2.5] (Phycoerythrin)
    See all Bcl-XL primary antibodies
  • Description
    Mouse monoclonal [7B2.5] to Bcl-XL (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab26035 recognises Bcl-xL.
  • Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Bcl-XL.
    Database link: Q07817

Applications

Our Abpromise guarantee covers the use of ab26035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.3µg for 106 cells. ab91541-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.

Target

  • FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

References for Anti-Bcl-XL antibody [7B2.5] (Phycoerythrin) (ab26035)

This product has been referenced in:
  • Khare A  et al. Role of Bruton's tyrosine kinase in macrophage apoptosis. Apoptosis 16:334-46 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21193961) »
  • Henderson RB  et al. A novel Rac-dependent checkpoint in B cell development controls entry into the splenic white pulp and cell survival. J Exp Med 207:837-53 (2010). Flow Cyt ; Mouse . Read more (PubMed: 20308364) »

See all 4 Publications for this product

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We do not have the detailed protocol that you requested, however please find attached our Abcam protocols book that contains lots of staining protocols and tips.

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Thank you for your enquiry. Suitable isotype controls for mouse monoclonal [7B2.5] to Bcl xL (Phycoerythrin) (ab26035) (IgG3) for FACS are: ab18391 Mouse IgG3, kappa FITC conjugated ab18392 Mouse IgG3, kappa FACS, IF, IHC-Fr, IHC-P, IP, WB ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"