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Our Abpromise guarantee covers the use of ab2568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.Can be blocked with Human Bcl-XL peptide (ab52114).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ICC||Use at an assay dependent concentration.|
ab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin
ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody.
Lane 1: Immunoprecipitated material
Lane 2: Control lane (no antibody)
This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006