Overview

  • Product nameAnti-Bcl-XL antibody
    See all Bcl-XL primary antibodies
  • Description
    Rabbit polyclonal to Bcl-XL
  • SpecificityAb47123 recognizes Bcl-XL .
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Gerbil
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-XL aa 61-79.
    Sequence:

    DSPAVNGATAHSSSLDARE


    Database link: Q07817

  • Positive control
    • CNS lymphatic tissues, many cancer cell lines

Applications

Our Abpromise guarantee covers the use of ab47123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000.
WB 1/1000 - 1/2000. Predicted molecular weight: 26 kDa.
IHC-Fr Use at an assay dependent concentration.
IP 1/50 - 1/200.

Target

  • FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody images

  • Formalin fixed paraffin embedded section of dog ischemic brain cortex stained for Bcl-XL expression using ab47123 at 1/2000. At 2 hr post ischemia, Bcl-XL staining was seen in the dying neurons that had morphological features of apoptosis (arrows). In contrast, mophologically normal appearing neurons lacked Bcl-X staining (arrowheads). Hematoxylin-eosin counterstain.

  • Formalin fixed paraffin embedded human tissue sections stained for Bcl-XL expression using ab47123 at 1/2000. A. Cortex from the brain of an Alzheimer's disease patient. The degenerating neurons are positive for Bcl-XL expression, the intact neurons are not. B. Normal bone marrow. The cells positive for Bcl-XLexpression appear to be of erythropoetic lineage. Hematoxylin-eosin counterstain.

References for Anti-Bcl-XL antibody (ab47123)

This product has been referenced in:
  • Loughran ST  et al. Bfl-1 is a crucial pro-survival nuclear factor-?B target gene in Hodgkin/Reed-Sternberg cells. Int J Cancer 129:2787-96 (2011). Read more (PubMed: 21491422) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"