Overview

  • Product nameAnti-Bcl-XL antibody, prediluted
    See all Bcl-XL primary antibodies
  • Description
    Rabbit polyclonal to Bcl-XL, prediluted
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • Colon carcinoma, Hodgkin's Lymphoma - IHC 293T cell lysate - WB

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested working dilution of 1/4 (see PMID: 16299246). Heat mediated antigen retrieval may be required before commencing with IHC staining protocol.

Target

  • FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • Tissue specificityBcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • Cellular localizationMitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody, prediluted images

  • Anti-Bcl-XL antibody, prediluted (ab15276) at 1/100 dilution + 293T cell lysate

    Predicted band size : 26 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    The dilution stated here refers to the dilution used for a non-prediluted version of this product.
  • ab15276 staining human Bcl-XL in human Colon Carcinoma by Immunohistochemistry (FFPE-sections).

References for Anti-Bcl-XL antibody, prediluted (ab15276)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam

The concentration of ab15276, lot#* is 3.4 ug/ml, which is the same as the previous lot that you recieved.

Please do not hesitate to contact me with additional questions or concerns.

Thank you for contacting us. The concentration for both lots is 3.4 ug/ml. I hope this helps, please let me know if you need any additional information.

This antibody was prediluted to a concentration of 4ug/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"