The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/25 - 1/100 in an ABC method. Antibody incubation period of 30 minutes at room temperature is recommended. Perform heat mediated antigen retrieval with 10mM citrate buffer, pH 6.0 before commencing with IHC staining protocol.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. Note=Defects in BCL10 are involved in various types of cancer.
Sequence similaritiesContains 1 CARD domain.
Post-translational modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.