Anti-Bcl10 antibody [4F8E8H8] (ab36585)


  • Product nameAnti-Bcl10 antibody [4F8E8H8]
    See all Bcl10 primary antibodies
  • Description
    Mouse monoclonal [4F8E8H8] to Bcl10
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • MCF-7 cell extract. Human breast and liver carcinoma tissue.


Associated products


Our Abpromise guarantee covers the use of ab36585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/1000.
WB 1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
ICC/IF 1/200 - 1/1000.


  • FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similaritiesContains 1 CARD domain.
  • Post-translational
    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI132454 antibody
    • B cell CLL/lymphoma 10 antibody
    • B cell lymphoma/leukemia10 antibody
    • B-cell CLL/lymphoma 10 antibody
    • B-cell leukemia/lymphoma 10 antibody
    • B-cell lymphoma/leukemia 10 antibody
    • Bcl 10 antibody
    • Bcl-10 antibody
    • Bcl10 antibody
    • BCL10_HUMAN antibody
    • c E10 antibody
    • c-E10 antibody
    • C81403 antibody
    • CARD containing apoptotic signaling protein antibody
    • CARD containing molecule enhancing NF kappa B antibody
    • CARD containing molecule enhancing NF kB antibody
    • CARD containing molecule enhancing NF-kB antibody
    • CARD containing molecule enhancing NFkB antibody
    • CARD containing proapoptotic protein antibody
    • CARD like apoptotic protein antibody
    • CARD-containing apoptotic signaling protein antibody
    • CARD-containing molecule enhancing NF-kappa-B antibody
    • CARD-containing proapoptotic protein antibody
    • CARD-like apoptotic protein antibody
    • CARMEN antibody
    • Caspase recruiting domain containing protein antibody
    • caspase-recruiting domain-containing protein antibody
    • cCARMEN antibody
    • cE 10 antibody
    • cE10 antibody
    • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
    • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
    • CED3/ICH1 prodomain homologous E10 like regulator antibody
    • Cellular E10 antibody
    • Cellular homolog of vCARMEN antibody
    • Cellular-E10 antibody
    • CIPER antibody
    • CLAP antibody
    • hCLAP antibody
    • Mammalian CARD containing adapter molecule E10 antibody
    • Mammalian CARD-containing adapter molecule E10 antibody
    • mE 10 antibody
    • mE10 antibody
    • R-RCD1 antibody
    see all

Anti-Bcl10 antibody [4F8E8H8] images

  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
  • All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

    Lane 1 : Cell lysates prepared from NIH/3T3 cells
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Cell lysates prepared from MCF-7 cells
    Lane 4 : Cell lysates prepared from Jurkat cells

    Lysates/proteins at 100 µg per lane.

    HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size : 31 kDa
  • ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

  • Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

References for Anti-Bcl10 antibody [4F8E8H8] (ab36585)

ab36585 has not yet been referenced specifically in any publications.

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The antibody ab36585 was raised agianst the Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3) - thus we do not know where the epitope region is and whether it is N- or C-terminal.

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