Anti-Bcl10 antibody [4F8E8H8] (ab36585)

Overview

  • Product nameAnti-Bcl10 antibody [4F8E8H8]
    See all Bcl10 primary antibodies
  • Description
    Mouse monoclonal [4F8E8H8] to Bcl10
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

  • Positive control
    • MCF-7 cell extract. Human breast and liver carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab36585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/1000.
WB 1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
ICC/IF 1/200 - 1/1000.

Target

  • FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similaritiesContains 1 CARD domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI132454 antibody
    • B cell CLL/lymphoma 10 antibody
    • B cell lymphoma/leukemia10 antibody
    • B-cell CLL/lymphoma 10 antibody
    • B-cell leukemia/lymphoma 10 antibody
    • B-cell lymphoma/leukemia 10 antibody
    • Bcl 10 antibody
    • Bcl-10 antibody
    • Bcl10 antibody
    • BCL10_HUMAN antibody
    • c E10 antibody
    • c-E10 antibody
    • C81403 antibody
    • CARD containing apoptotic signaling protein antibody
    • CARD containing molecule enhancing NF kappa B antibody
    • CARD containing molecule enhancing NF kB antibody
    • CARD containing molecule enhancing NF-kB antibody
    • CARD containing molecule enhancing NFkB antibody
    • CARD containing proapoptotic protein antibody
    • CARD like apoptotic protein antibody
    • CARD-containing apoptotic signaling protein antibody
    • CARD-containing molecule enhancing NF-kappa-B antibody
    • CARD-containing proapoptotic protein antibody
    • CARD-like apoptotic protein antibody
    • CARMEN antibody
    • Caspase recruiting domain containing protein antibody
    • caspase-recruiting domain-containing protein antibody
    • cCARMEN antibody
    • cE 10 antibody
    • cE10 antibody
    • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
    • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
    • CED3/ICH1 prodomain homologous E10 like regulator antibody
    • Cellular E10 antibody
    • Cellular homolog of vCARMEN antibody
    • Cellular-E10 antibody
    • CIPER antibody
    • CLAP antibody
    • hCLAP antibody
    • Mammalian CARD containing adapter molecule E10 antibody
    • Mammalian CARD-containing adapter molecule E10 antibody
    • mE 10 antibody
    • mE10 antibody
    • R-RCD1 antibody
    see all

Anti-Bcl10 antibody [4F8E8H8] images

  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
  • Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
  • All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

    Lane 1 : Cell lysates prepared from NIH/3T3 cells
    Lane 2 : Cell lysates prepared from Hela cells
    Lane 3 : Cell lysates prepared from MCF-7 cells
    Lane 4 : Cell lysates prepared from Jurkat cells

    Lysates/proteins at 100 µg per lane.

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size : 31 kDa
  • ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

  • Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

References for Anti-Bcl10 antibody [4F8E8H8] (ab36585)

ab36585 has not yet been referenced specifically in any publications.

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The antibody ab36585 was raised agianst the Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3) - thus we do not know where the epitope region is and whether it is N- or C-terminal.
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