Overview

  • Product nameAnti-Bcl10 antibody [EP606Y]
    See all Bcl10 primary antibodies
  • Description
    Rabbit monoclonal [EP606Y] to Bcl10
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bcl10 aa 200 to the C-terminus (C terminal).

  • Positive control
    • WB: Raji cell lysate, Hela cell lysate IHC: Human hepatocellular and lungcarcinoma ICC/IF: Raji cells IP: Ramos cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Bcl10 antibody (Alexa Fluor® 488) [EP606Y] (ab200318)

    Anti-Bcl10 antibody (Alexa Fluor® 647) [EP606Y] (ab200320)
    Anti-Bcl10 antibody (HRP) [EP606Y] (ab200319)

Properties

Applications

Our Abpromise guarantee covers the use of ab33905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/50.
IP 1/20 - 1/50.

Target

  • FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similaritiesContains 1 CARD domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI132454 antibody
    • B cell CLL/lymphoma 10 antibody
    • B cell lymphoma/leukemia10 antibody
    • B-cell CLL/lymphoma 10 antibody
    • B-cell leukemia/lymphoma 10 antibody
    • B-cell lymphoma/leukemia 10 antibody
    • Bcl 10 antibody
    • Bcl-10 antibody
    • Bcl10 antibody
    • BCL10_HUMAN antibody
    • c E10 antibody
    • c-E10 antibody
    • C81403 antibody
    • CARD containing apoptotic signaling protein antibody
    • CARD containing molecule enhancing NF kappa B antibody
    • CARD containing molecule enhancing NF kB antibody
    • CARD containing molecule enhancing NF-kB antibody
    • CARD containing molecule enhancing NFkB antibody
    • CARD containing proapoptotic protein antibody
    • CARD like apoptotic protein antibody
    • CARD-containing apoptotic signaling protein antibody
    • CARD-containing molecule enhancing NF-kappa-B antibody
    • CARD-containing proapoptotic protein antibody
    • CARD-like apoptotic protein antibody
    • CARMEN antibody
    • Caspase recruiting domain containing protein antibody
    • caspase-recruiting domain-containing protein antibody
    • cCARMEN antibody
    • cE 10 antibody
    • cE10 antibody
    • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
    • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
    • CED3/ICH1 prodomain homologous E10 like regulator antibody
    • Cellular E10 antibody
    • Cellular homolog of vCARMEN antibody
    • Cellular-E10 antibody
    • CIPER antibody
    • CLAP antibody
    • hCLAP antibody
    • Mammalian CARD containing adapter molecule E10 antibody
    • Mammalian CARD-containing adapter molecule E10 antibody
    • mE 10 antibody
    • mE10 antibody
    • R-RCD1 antibody
    see all

Anti-Bcl10 antibody [EP606Y] images

  • All lanes : Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/5000 dilution (purified)

    Lane 1 : Raji cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : HuT-78 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 31 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/5000 dilution (unpurified) + Raji cell lysate

    Predicted band size : 31 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/1000 dilution (unpurified) + Recombinant Human Bcl10 protein (ab82241) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa


    Exposure time : 1 minute
  • Immunofluorescence staining of Raji cells with purified ab33905 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab33905 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab33905 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Unpurified ab33905, at a 1/100 dilution, staining human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.

    ab33905, at a 1/100 dilution, staining human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.
  • Unpurified ab33905, staining Hela cells by Immunofluorescent.

    ab33905, staining Hela cells by Immunofluorescent.
  • ab33905 (purified) at 1/20 immunoprecipitating Bcl10 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 32 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

References for Anti-Bcl10 antibody [EP606Y] (ab33905)

This product has been referenced in:
  • Meininger I  et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Read more (PubMed: 27068814) »
  • Mc Guire C  et al. Pharmacological inhibition of MALT1 protease activity protects mice in a mouse model of multiple sclerosis. J Neuroinflammation 11:124 (2014). WB ; Mouse . Read more (PubMed: 25043939) »

See all 2 Publications for this product

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