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Overview

  • Product name
    Anti-Bcl10 antibody [EP606Y]
    See all Bcl10 primary antibodies
  • Description
    Rabbit monoclonal [EP606Y] to Bcl10
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bcl10 aa 200 to the C-terminus (C terminal).

  • Positive control
    • WB: Raji and HeLa cell lysate. IHC-P: Human hepatocellular and lungcarcinoma tissues. ICC/IF: Raji and HeLa cells. IP: Ramos cell lysate. Flow Cyt: Raji cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Bcl10 antibody (Alexa Fluor® 488) [EP606Y] (ab200318)

    Anti-Bcl10 antibody (Alexa Fluor® 647) [EP606Y] (ab200320)
    Anti-Bcl10 antibody (HRP) [EP606Y] (ab200319)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab33905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/50.
IP 1/20 - 1/50.

Target

  • Function
    Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
    Note=Defects in BCL10 are involved in various types of cancer.
  • Sequence similarities
    Contains 1 CARD domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
  • Cellular localization
    Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
    • Alternative names
      • AI132454 antibody
      • B cell CLL/lymphoma 10 antibody
      • B cell lymphoma/leukemia10 antibody
      • B-cell CLL/lymphoma 10 antibody
      • B-cell leukemia/lymphoma 10 antibody
      • B-cell lymphoma/leukemia 10 antibody
      • Bcl 10 antibody
      • Bcl-10 antibody
      • Bcl10 antibody
      • BCL10_HUMAN antibody
      • c E10 antibody
      • c-E10 antibody
      • C81403 antibody
      • CARD containing apoptotic signaling protein antibody
      • CARD containing molecule enhancing NF kappa B antibody
      • CARD containing molecule enhancing NF kB antibody
      • CARD containing molecule enhancing NF-kB antibody
      • CARD containing molecule enhancing NFkB antibody
      • CARD containing proapoptotic protein antibody
      • CARD like apoptotic protein antibody
      • CARD-containing apoptotic signaling protein antibody
      • CARD-containing molecule enhancing NF-kappa-B antibody
      • CARD-containing proapoptotic protein antibody
      • CARD-like apoptotic protein antibody
      • CARMEN antibody
      • Caspase recruiting domain containing protein antibody
      • caspase-recruiting domain-containing protein antibody
      • cCARMEN antibody
      • cE 10 antibody
      • cE10 antibody
      • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
      • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
      • CED3/ICH1 prodomain homologous E10 like regulator antibody
      • Cellular E10 antibody
      • Cellular homolog of vCARMEN antibody
      • Cellular-E10 antibody
      • CIPER antibody
      • CLAP antibody
      • hCLAP antibody
      • Mammalian CARD containing adapter molecule E10 antibody
      • Mammalian CARD-containing adapter molecule E10 antibody
      • mE 10 antibody
      • mE10 antibody
      • R-RCD1 antibody
      see all

    Anti-Bcl10 antibody [EP606Y] images

    • Western blot - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Western blot - Anti-Bcl10 antibody [EP606Y] (ab33905)
      All lanes : Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/5000 dilution (purified)

      Lane 1 : Raji cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : HuT-78 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 31 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)

      Immunofluorescence staining of Raji cells with purified ab33905 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab33905 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab33905 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Flow Cytometry - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Flow Cytometry - Anti-Bcl10 antibody [EP606Y] (ab33905)

      Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling Bcl10 (red) with ab33905 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • Immunoprecipitation - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunoprecipitation - Anti-Bcl10 antibody [EP606Y] (ab33905)
      ab33905 (purified) at 1/20 immunoprecipitating Bcl10 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 32 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Western blot
      Western blot
      Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/1000 dilution (unpurified) + Recombinant Human Bcl10 protein (ab82241) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 31 kDa


      Exposure time : 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)

      Unpurified ab33905, at a 1/100 dilution, staining human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.

    • Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)
      Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)

      Unpurified ab33905, staining HeLa cells by Immunofluorescent.

    References for Anti-Bcl10 antibody [EP606Y] (ab33905)

    This product has been referenced in:
    • Ginster S  et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . Read more (PubMed: 28052131) »
    • Meininger I  et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Read more (PubMed: 27068814) »

    See all 3 Publications for this product

    Publishing research using ab33905? Please let us know so that we can cite the reference in this datasheet.

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