Anti-Bcl10 antibody [EP606Y] (ab33905)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-Bcl10 antibody [EP606Y]
See all Bcl10 primary antibodies -
DescriptionRabbit monoclonal [EP606Y] to Bcl10
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-P, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bcl10 aa 200 to the C-terminus (C terminal).
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Positive control
- WB: Raji and HeLa cell lysate. IHC-P: Human hepatocellular and lungcarcinoma tissues. ICC/IF: Raji and HeLa cells. IP: Ramos cell lysate. Flow Cyt: Raji cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP606Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab33905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/200. | |
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa). | |
| IHC-P | 1/50. | |
| IP | 1/20 - 1/50. |
Target
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FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
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Tissue specificityUbiquitous.
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Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
Sequence similaritiesContains 1 CARD domain.
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Post-translational
modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
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Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
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Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Images
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![Western blot - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-259959-ab33905WB1.jpg)
Western blot - Anti-Bcl10 antibody [EP606Y] (ab33905)All lanes : Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/5000 dilution (purified)
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 32 kDa (why is the actual band size different from the predicted?)Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-7-ab33905ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)Immunofluorescence staining of Raji cells with purified ab33905 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab33905 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-6-ab33905IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab33905 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Flow Cytometry - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-10-ab33905FC1.jpg)
Flow Cytometry - Anti-Bcl10 antibody [EP606Y] (ab33905)Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling Bcl10 (red) with ab33905 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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![Immunoprecipitation - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-8-ab33905IP1.jpg)
Immunoprecipitation - Anti-Bcl10 antibody [EP606Y] (ab33905)ab33905 (purified) at 1/20 immunoprecipitating Bcl10 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 32 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST -
Western blotAnti-Bcl10 antibody [EP606Y] (ab33905) at 1/1000 dilution (unpurified) +Recombinant Human Bcl10 protein (ab82241) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Exposure time: 1 minute -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EP606Y] (ab33905)Unpurified ab33905, at a 1/100 dilution, staining human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.
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![Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)](http://a.abcam.com/ps/products/33/ab33905/Images/ab33905-3.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Bcl10 antibody [EP606Y] (ab33905)Unpurified ab33905, staining HeLa cells by Immunofluorescent.
References
This product has been referenced in:
- Ginster S et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . Read more (PubMed: 28052131) »
- Meininger I et al. Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells. Nat Commun 7:11292 (2016). Mouse . Read more (PubMed: 27068814) »
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