Overview

  • Product name
    Anti-Bcl10 antibody [EPR3175]
    See all Bcl10 primary antibodies
  • Description
    Rabbit monoclonal [EPR3175] to Bcl10
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Bcl10.

  • Positive control
    • Daudi, Raji, HeLa, and HuT-78 cell lysates; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
    • Tissue specificity
      Ubiquitous.
    • Involvement in disease
      Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
      Note=Defects in BCL10 are involved in various types of cancer.
    • Sequence similarities
      Contains 1 CARD domain.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
    • Cellular localization
      Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI132454 antibody
      • B cell CLL/lymphoma 10 antibody
      • B cell lymphoma/leukemia10 antibody
      • B-cell CLL/lymphoma 10 antibody
      • B-cell leukemia/lymphoma 10 antibody
      • B-cell lymphoma/leukemia 10 antibody
      • Bcl 10 antibody
      • Bcl-10 antibody
      • Bcl10 antibody
      • BCL10_HUMAN antibody
      • c E10 antibody
      • c-E10 antibody
      • C81403 antibody
      • CARD containing apoptotic signaling protein antibody
      • CARD containing molecule enhancing NF kappa B antibody
      • CARD containing molecule enhancing NF kB antibody
      • CARD containing molecule enhancing NF-kB antibody
      • CARD containing molecule enhancing NFkB antibody
      • CARD containing proapoptotic protein antibody
      • CARD like apoptotic protein antibody
      • CARD-containing apoptotic signaling protein antibody
      • CARD-containing molecule enhancing NF-kappa-B antibody
      • CARD-containing proapoptotic protein antibody
      • CARD-like apoptotic protein antibody
      • CARMEN antibody
      • Caspase recruiting domain containing protein antibody
      • caspase-recruiting domain-containing protein antibody
      • cCARMEN antibody
      • cE 10 antibody
      • cE10 antibody
      • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
      • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
      • CED3/ICH1 prodomain homologous E10 like regulator antibody
      • Cellular E10 antibody
      • Cellular homolog of vCARMEN antibody
      • Cellular-E10 antibody
      • CIPER antibody
      • CLAP antibody
      • hCLAP antibody
      • Mammalian CARD containing adapter molecule E10 antibody
      • Mammalian CARD-containing adapter molecule E10 antibody
      • mE 10 antibody
      • mE10 antibody
      • R-RCD1 antibody
      see all

    Images

    • All lanes : Anti-Bcl10 antibody [EPR3175] (ab108328) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HuT-78 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa
    • ab108328, at 1/100 dilution, staining Bcl10 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Torres JM  et al. Inherited BCL10 deficiency impairs hematopoietic and nonhematopoietic immunity. J Clin Invest 124:5239-48 (2014). Read more (PubMed: 25365219) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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