Anti-Bcl2 alpha antibody [100/D5] (ab77567)


  • Product nameAnti-Bcl2 alpha antibody [100/D5]
    See all Bcl2 alpha primary antibodies
  • Description
    Mouse monoclonal [100/D5] to Bcl2 alpha
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 41-54 of Human Bcl2 alpha

  • Positive control
    • Raji Cells. Tonsil or follicular lymphoma tissue.



Our Abpromise guarantee covers the use of ab77567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Incubation 2hrs at room temperature
IP Use at 2 µg/mg of lysate.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.


  • RelevanceBCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
  • Cellular localizationMitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Apoptosis regulator Bcl2 antibody
    • B cell lymphoma protein 2 alpha isoform antibody
    • B cell lymphoma protein 2 antibody
    • B-cell CLL/lymphoma 2 antibody
    • B-cell leukemia/lymphoma 2 antibody
    • Bcl 2 antibody
    • Leukemia /lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    see all

Anti-Bcl2 alpha antibody [100/D5] images

  • Formalin-fixed, paraffin-embedded human tonsil with 2-4ug/ml ab77567, using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of follicular cells and no staining of reactive cells in the germinal center.
  • Anti-Bcl2 alpha antibody [100/D5] (ab77567) at 1 µg/ml + Human lymphoma cells lysate

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Overlay histogram showing HepG2 cells stained with ab77567 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77567, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Bcl2 alpha antibody [100/D5] (ab77567)

ab77567 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc1 pancreatic cancer cell line)
Permeabilization Yes - 0.25% TritonX-100 in PBS
Specification Panc1 pancreatic cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde

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Submitted Jan 19 2016